Adaptation of in vitro methodologies to estimate the intestinal digestion of lipids in ruminants

Abstract The objective of this study was to adapt existing in vitro methodologies determine the extent intestinal digestion corn oil (CO), canola (CA), and beef tallow (BT) via manipulation incubation length concentrations lipase, bile, calcium within a buffer solution. Unless otherwise stated, 0.5 g each lipid source were incubated separately triplicate, with triplicate batch culture runs for treatment 40 mL M KH2PO4 (pH = 7.6) 24 h pancreatin (8 g/L), bovine bile (2.5 CaCl2 (10 mM). Individually, pancreatin, CaCl2, as well tested. To examine use assay estimate total tract digestion, solution concentrated amounts reach same final Ca used third step three-step digestibility procedure. Free glycerol free fatty acid (FFA) measured using colorimetric assays indicators digestion. Data wereanalyzed completely randomized block design (block run), Glimmix procedure SAS. For source, increased increasing pancreatin; however, FFA lowest at 0 g/L but similar 6, 8, 10 g/L. Both greater 2.5 5 than source. Calcium concentration did not affect or either CO CA; BT when included mM mM. all fat sources, after 1 until 12 h, increase from h. When mixture following fermentation acidification steps, compared just adding buffer; indeterminable. Thus, can be is least containing 8 only estimating digestion; utilizing procedure, used.